o. van-ham (ovanham at julian.uwo.ca) wrote:
: I had a similar prooblem some time ago. It drove me nuts; I'd have a
: beautiful gel that would fall apart when I'd separate the plates. The
: problem turned out to be that I wasn't letting the gel sit long enough
: after pouring. I would pour the gel first thing in the morning, and run
: the gel early afternoon. If you're not already doing so, I'd recommend
: pouring the gel at the end of the day, and running the gel in the
: morning. This seems to give the gel enough time to adhere properly to
: the bind-silane coated plate. Good luck.
I don't think this is the problem. I have run my sqcing gels even 3hrs after
I poured them. The problem might lie in:
1) Unclean plates.
2) not treating one of the plates.
3) Cheap Whatman 3MM paper.
C A N A D A
Program in Molecular Biology
Department of Chemistry and Biochemistry Box 3C
New Mexico State University
Las Cruces NM