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CAT assay - best method?

bbraun at ucla.edu bbraun at ucla.edu
Fri Nov 24 05:31:10 EST 1995

I'm sure this is a common question, so FAQ pointers are welcome.

What is the best way do do CAT assays?
Concerns include:

1. sensitivity
2. reproducibility
3. convenience - e.g. 50 samples or more in one sitting
4. compatibility of lysate with B-gal or Luciferase assays
5. anything else?

Note that cost, although important, is not a priority.  I am
currently leaning towards an ELISA kit like the one
Boehrringer sells.

Any suggestions?

Benjamin Braun

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