BSA in PCR

Benny Shomer bshomer at ebi.ac.uk
Fri Nov 24 09:27:42 EST 1995


Edward Wang (ez022056 at dale.ucdavis.edu) wrote:
: What is the purpose of BSA in PCR, and what grade of BSA should one use 
: for the PCR buffer?


The purpose of BSA is to stabilize the polymerase. Some suggest that it is
not allways needed. The neccesity of BSA depends on the type of polymerase
used.  I highly recommend the molecular grade BSA (normally provided when
purchasing restriction enzymes). Other preparations often contain DNAses
and inhibitors.

Hope this helps,

Benny.

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~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Benny Shomer
External Biological Liaison Officer,
EMBL outstation - The EBI,
Hinxton Hall, Hinxton,Cambridge CB10 1RQ, UK

Tel:   +44-223-494437
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