IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

Digestion at the termini of PCR product?

Zheng Huanquan imazhq at leonis.nus.sg
Sat Nov 25 01:57:58 EST 1995

I currently get a PCR product which has Nhe I at 5' terminus and BamH I 
at 3'. I would like to ligate it into pET21a. Any one has experience in 
digesting DNA with enzymes which sites are at termini? any protocol?


H Zheng

More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net