Digestion at the termini of PCR product?

Zheng Huanquan imazhq at leonis.nus.sg
Sat Nov 25 01:57:58 EST 1995


I currently get a PCR product which has Nhe I at 5' terminus and BamH I 
at 3'. I would like to ligate it into pET21a. Any one has experience in 
digesting DNA with enzymes which sites are at termini? any protocol?

Thanks!


H Zheng





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