DNA "ghost" bands after isolation

Margon Vandongen vando005 at mc.duke.edu
Sat Nov 25 10:21:55 EST 1995


Daniel,

I assume you use the geneclean method or something similar. I have
seen this too when I ran the DNA out too fast after eluting 
the fragment off the beads. I think it might be single strands that
have not annealed back yet? When I reran the same preparation a half
hour later the DNA looked fine.Alternatively if you work with large
fragments you might actually be shearing your DNA. Hope this helps,

Margon
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