Up to now we got big problems by transient transfection of primary
macrophages and murine or human macrophage cellines with CAT reporter
constructs. We are using the DEAE-Dextran method (Current protocols). Is
anybody able to give hints for increasing the efficiency of transfecting
I'm glad for any good answer.
Thank you and best regards
Dr Dirk Seegert
Fraunhofer Institut Hannover