transfection

[joerg seegert]

Up to now we got big problems by transient transfection of primary 
macrophages and murine or human macrophage cellines with CAT reporter 
constructs. We are using the DEAE-Dextran method (Current protocols). Is 
anybody able to give hints for increasing the efficiency of transfecting 
these cells?
I'm glad for any good answer.
Thank you and best regards

Dr Dirk Seegert
Fraunhofer Institut Hannover