GIBCO M-MLv RT
smori at nmsu.edu
Sat Nov 25 22:48:12 EST 1995
hfakhour (hfakhour at fs1.ho.man.ac.uk) wrote:
: Hi !
: We recently changed from using a kit for reverse transcription into using GIBCO
: M-MLv reverse transcriptase. We have been trying to sort out a protocol for
: that, but so far no success. The primers we use are random hexamers, our
: starting material is total RNA (1-2 ug), we do not use any labelled dNTP. The
: resulting cDNA is used for PCR. Could anyone please suggest a succesful
: Thanking you in advance
Try their SuperScript II enzyme. I have had fairly good luck with.
Has anybody else had consistency problems with RT's from GIBCO? Do different
batches work differently for you????
C A N A D A
Program in Molecular Biology
Department of Chemistry and Biochemistry Box 3C
New Mexico State University
Las Cruces NM
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