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Shahram Mori smori at nmsu.edu
Sat Nov 25 22:48:12 EST 1995

hfakhour (hfakhour at fs1.ho.man.ac.uk) wrote:

: Hi !

: We recently changed from using a kit for reverse transcription into using GIBCO 
: M-MLv reverse transcriptase. We have been trying to sort out a protocol for 
: that, but so far no success.  The primers we use are random hexamers, our 
: starting material is  total RNA (1-2 ug), we do not use any labelled dNTP. The 
: resulting cDNA is used for PCR. Could anyone please suggest a succesful 
: protocol.

: Thanking you in advance

: Hannah

Try their SuperScript II enzyme. I have had fairly good luck with.

Has anybody else had consistency problems with RT's from GIBCO? Do different
batches work differently for you????
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			C   A   N   A   D   A
Shahram Mori
Program in Molecular Biology
Department of Chemistry and Biochemistry Box 3C
New Mexico State University
Las Cruces NM

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