Immuno detection of T-cells ?.

[Dima Klenchin]

In article <49a17o$176 at fs2.abdn.ac.uk>, gen154 at abdn.ac.uk (a.molloy) wrote:
>
>Hi
>

>not sure what the best way is. I tried to do an ELISA but the
>cells wont stick to the plate. 

Poly-L-Lysine is the answer. 100 ug/ml / well - 1 hr, 37C --> wash 3X -->
add cells. Mystery if they won't. 

- Dima