Immuno detection of T-cells ?.

Dima Klenchin klenchin at macc.wisc.edu
Sun Nov 26 19:57:24 EST 1995


In article <49a17o$176 at fs2.abdn.ac.uk>, gen154 at abdn.ac.uk (a.molloy) wrote:
>
>Hi
>

>not sure what the best way is. I tried to do an ELISA but the
>cells wont stick to the plate. 

Poly-L-Lysine is the answer. 100 ug/ml / well - 1 hr, 37C --> wash 3X -->
add cells. Mystery if they won't. 

- Dima




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