I am having considerable trouble with the Altered Sites II system from
Promega. My problems are centered around the R408 helper phage. ALL of
the colonies that I get after using the system are contaminated with the
R408 helper phage and it is impossible for me to use any of my mutants.
When I try to grow up the colonies for sequencing or cloning I get an over
growth of R408 which makes the plasmid produce ssDNA instead of clonable
DNA. I was wondering if anyone else had experience with this kit and if
so have you had any problems with it.
Thanks in advance,
Molecular Microbiology and Immunology
University of Missouri-Columbia
C601591 at showme.missouri.edu