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Altered Sites II

Don Haut c601591 at mizzou1.missouri.edu
Sun Nov 26 11:21:59 EST 1995

I am having considerable trouble with the Altered Sites II system from
Promega.  My problems are centered around the R408 helper phage.  ALL of
the colonies that I get after using the system are contaminated with the
R408 helper phage and it is impossible for me to use any of my mutants. 
When I try to grow up the colonies for sequencing or cloning I get an over
growth of R408 which makes the plasmid produce ssDNA instead of clonable
DNA.  I was wondering if anyone else had experience with this kit and if
so have you had any problems with it.

Thanks in advance,


Don Haut 
Molecular Microbiology and Immunology
University of Missouri-Columbia
C601591 at showme.missouri.edu

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