transfection

[martin LEACH]

joerg seegert (joerg.seegert at flensburg.netsurf.de) wrote:
: Up to now we got big problems by transient transfection of primary 
: macrophages and murine or human macrophage cellines with CAT reporter 
: constructs. We are using the DEAE-Dextran method (Current protocols). Is 
: anybody able to give hints for increasing the efficiency of transfecting 
: these cells?

I believe glycerol or DMSO shocking of the cells increases transfection
efficiency...this works with capo4 method....not sure about dextran
method....take a look in maniatis/sambrook

Martin

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