Cloning receptor genes using the ligand?

N.P.Dantuma at biol.ruu.nl N.P.Dantuma at biol.ruu.nl
Mon Nov 27 11:06:50 EST 1995


In article <49079m$rfb at news.cais.com> rover at ns1.livnet.com (Rover) writes:
>From: rover at ns1.livnet.com (Rover)
>Subject: Cloning receptor genes using the ligand?
>Date: Thu, 23 Nov 1995 01:02:54 GMT


>        We have heard in the wind  that there is a method somewhere for
>cloning receptor genes using the ligand or ligand information. If
>anyone has heard of this or knows of a reference please let me know.

If this receptor is a cell-surface receptor you can use the panning method.  
This method is described in (for example) EMBO 11: 3313-3316. I think that 
the yeast two hybrid system is only useful if the receptor is a cytoplasmic 
receptor. As mentioned in other replies I would also like to stress that the 
characterization of the binding is very essential. The panning method have 
been used for the cloning of several receptors and is, I think, less 
laborious then tagging receptor-expressing cells with radioligand.

Nico Dantuma



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