In article <49079m$rfb at news.cais.com> rover at ns1.livnet.com (Rover) writes:
>From: rover at ns1.livnet.com (Rover)
>Subject: Cloning receptor genes using the ligand?
>Date: Thu, 23 Nov 1995 01:02:54 GMT
> We have heard in the wind that there is a method somewhere for
>cloning receptor genes using the ligand or ligand information. If
>anyone has heard of this or knows of a reference please let me know.
If this receptor is a cell-surface receptor you can use the panning method.
This method is described in (for example) EMBO 11: 3313-3316. I think that
the yeast two hybrid system is only useful if the receptor is a cytoplasmic
receptor. As mentioned in other replies I would also like to stress that the
characterization of the binding is very essential. The panning method have
been used for the cloning of several receptors and is, I think, less
laborious then tagging receptor-expressing cells with radioligand.