Q:baculovirus infecting HepG2 cells

Richard Vernell rjv at uhura.neoucom.edu
Mon Nov 27 10:46:01 EST 1995


M K Archer (fr80 at dial.pipex.com) wrote:
: The reference you are after is 
:               
:         PNAS Vol92 (22) pp10099-10103
:    
:   Efficient Gene Transfer into Human Hepatocytes by Bacculovirus Vectors.

:          Hope you can find it.
:  

Baculovirus is not infecting the hepatoma cells.  The gene of interest is
cloned into the baculovirus genome.  Packaging the DNA in virus particles
is facilitating the uptake and delivery to the nucleus.  As I understand
it, viral genes are not expressed.  

Rich Vernell

rjv at uhura.neoucom.edu



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