Forwarded: grinding medium

Jenny Davila-Aponte 23144jda at msu.edu
Mon Nov 27 17:17:54 EST 1995


I'm a post-doc in Ken Keegstra's lab, where we study import of proteins
into chloroplasts.  The grinding buffer we use contains the following:
	50 mM HEPES-KOH, pH 7.3
	330 mM sorbitol
	0.1% bovine serum albumin, fraction V
	1 mM MgCl2
	1 mM MnCl2
	2 mM Na2EDTA

We don't normally include glutathione or isoascorbate, but if they're
added, we don't bother to adjust the pH after the addition.  

Hope this helps,
Jenny Davila-Aponte

-- 
Jenny Davila-Aponte
MSU-DOE Plant Research Laboratory
Michigan State University
23144jda at msu.edu



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