Forwarded: grinding medium

[THORN at LAN.TJHSST.EDU]

Dear netters,
Could anyone help this senior high school student?
Subject:       grinding medium
hello,
   I am making a grinding medium for the isolation of chloroplasts based 
on the reference, Cell Culture and Somatic Cell Genetics in 
Plants, volume 1, edited by Indra K. Vasil, chapter 54.  This medium 
contains sorbitol, magnesium chloride, pyrophosphate and isoascorbic 
acid.  After the medium was pH adjusted to 6.5, the isoascorbic acid? 
dropped out of solution and I have been unable to redissolve the 
precipitate.  Does anyone know whether or not the medium must have all 
components in solution?

    Thank you,
           Jessica Glasser
           jglasser at lan.tjhsst.edu
Toby Mogollon Horn, Ph.D.			*THE DNAHAND*   <thorn at pen.k12.va.us>
Life Science and Biotechnology Laboratory			<thorn at lan.tjhsst.edu>
Thomas Jefferson High School for Science and Technology
6560 Braddock Road
Alexandria, VA 22312	phone 703-750-5024		fax 703-750-5010