Measure E coli at O.D. 620nm

Alex Chang lpss at
Mon Nov 27 14:58:37 EST 1995

Following my question, I got the following responses:

John R. Rebers (jrebers at, Northern Michigan University): The 
measurement is based on light scattering by the bacteria, rather than 
light absorption, so I suspect the A620 measurement would come pretty 

Nico.Mertens at (Flemish Institute of Biotechnology): Some 
people also measure densities at 650, so I would use 620nm......

They both mentioned that standardisation is required.

Thanks very much.


Alex Chang
University of British Columbia
achang at

More information about the Methods mailing list