two-hybrid libraries

Kuan Chee Mun imakcm at leonis.nus.sg
Tue Nov 28 03:11:23 EST 1995


Hi all..

I'm currently making some GAL4-cDNA fusion libraries for two-hybrid
screening. Does anyone know how to control the RT reaction so that it's
efficient enough to to give decent first strands, and yet not hit the 5'
UTR ? (that'll mess up the fusion junction with GAL4 on the vector) 

Thanks a 10e6!

Chee Mun
Institute of Molecular Agrobiology
National University of Singapore

PS. Any tips to make sure I get all 3 frames in the right 5'-3' orientation?




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