Cloning receptor genes using the ligand?
juschus at novsrv1.pio1.uni-heidelberg.de
Tue Nov 28 11:34:20 EST 1995
In article <49aja7$3se at cwis-20.wayne.edu>, asg at cmb.biosci.wayne.edu says...
>bbraun at ucla.edu wrote:
>>>The yeast system is a no-no, I fear, since it will not work with surface
>>>membrane proteins, or ligands that are not proteins.
>I think Benjamin Braun is correct: the yeast two-hybrid system is not
>the way to go to find the receptor for an known ligand. I really do
>not think there are 'easy' paths to this cloning. I only think of the
>difficulty in cloning the TGFbeta receptor type II (1991) many years
>after the ligand (TGFbeta) was purified (1984) and cloned (1985). Phil
>Leder's group at Harvard Med succeeded in doing the other task: cloning
>the ligand (Steel factor) for the known receptor (c-kit); their paper
>is published in Cell in 1990. Their methodology can be generalized.
The two hybrid system was used however to clone a BMP receptor, published
in PNAS a few weeks back.
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