Cloning receptor genes using the ligand?

Kalifa juschus at
Tue Nov 28 11:34:20 EST 1995

In article <49aja7$3se at>, asg at says...
>bbraun at wrote:
>>>The yeast system is a no-no, I fear, since it will not work with surface 
>>>membrane proteins, or ligands that are not proteins.
>I think Benjamin Braun is correct:  the yeast two-hybrid system is not
>the way to go to find the receptor for an known ligand.  I really do
>not think there are 'easy' paths to this cloning.  I only think of the
>difficulty in cloning the TGFbeta receptor type II (1991) many years
>after the ligand (TGFbeta) was purified (1984) and cloned (1985).  Phil
>Leder's group at Harvard Med succeeded in doing the other task:  cloning
>the ligand (Steel factor) for the known receptor (c-kit); their paper
>is published in Cell in 1990.  Their methodology can be generalized.

The two hybrid system was used however to clone a BMP receptor, published
in PNAS a few weeks back.


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