In article <-2711951340000001 at mac6794.salk.edu>, @ wrote:
>I have been doing differential display reactions and get good gels and
>even amplify the bands out of the gel , But when I do northerns with
>reamplified bands there isn't any signal at all on the blots. I know
>RNA is fine and the transfer is fine too. Can't figure out what the
>Any help would be really appreciated.
>apotnis at aim.salk.edu
There is a review article in the October edition of Current Opinion in
Biotechnology on differential display. It may give you some clues on
why you can't detect any message on a northern. The main point of the
article appears to be that differential display is not all its cut out
to be, having a large number of difficulties. The complete reference
is "Christine Debouck: Differential display or differential dismay.
Current Opinion in Biotechnology 1995 Vol 6(5) pp597-599"
best of luck