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Tet Inducible Gene Expression

k mit.edu
Wed Nov 29 10:11:59 EST 1995

I am utilizing the Tet inducible (repressable) system for conditional gene
expression developed by Gossen and Bujard.  I've found that lines which
initially showed inducible expression of the desired gene eventually cease
to induce these genes after several passages.  Anyone else seen these? 
What causes this?  How do you prevent it?  Thanks


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