Agarose-DNA purification

Muhammad A. Lodhi lodhi at CSHL.ORG
Wed Nov 29 07:19:08 EST 1995


Amicon has columns for this puprpose.  Also Qiaex II gel extraction system
of Qiagen is probably the best that I have used.  Its simple, cheap and
recovery is quite high.

Muhammad A. Lodhi
Cols Spring Harbor Laboratory


>In article <48vagp$7pk at sparcserver.lrz-muenchen.de>, Tim Angelotti
><102055.606 at compuserve.com> wrote:
>
>> I have heard about microcentrifuge spin columns that can be used to
>purify DNA from agarose
>> gels.  These are not based on DNA binding matrix in the spin-column but
>are merely a rapid spin
>> that drives the gel solution and DNA out of the matrix.  Does anyone
>know who sells these or
>> how to make your own?
>
>I read in a recent issue of Biotechniques (1994 or 1995) about the use of
>filtered pipette tips as spin columns.  The authors claimed 50% recovery
>although I haven't tried it.
>
>Regards,
>
>Tim





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