neale at mbcf.stjude.org wrote:
>We are currently screening libraries in lambda gt10, and would like to check
>the size of the inserts (cDNA) by PCR. Inserts are cloned into the Eco RI site.
>Does anyone have sequences of primers (and PCR conditions if available) that
>work in lambda gt10?
>Thanks in advance,
>Dept. of Virology and Molecular Biology
>St. Jude Children's Research Hospital
>Phone: (901) 495-3400
>Fax: (901) 523-2622
>e-mail: geoffrey.neale at stjude.org>================================================================================
I've always just used the same primers sequences that NEB sells:
Hope this works for you.
mcelverja at phibred.com