SYBR Green I Dye for agarose gel eletroph. of DNA?

Your Name Here you at somehost.somedomain
Wed Nov 29 11:50:17 EST 1995


In article <49fesl$51c at news.acns.nwu.edu>, tar205 at nwu.edu (Tracey Ann Ristow Lozano) says:
>
>In article <Pine.A32.3.91.951125154854.79008B-100000 at cariari.ucr.ac.cr>, 
>japalaci at cariari.ucr.ac.cr says...
>>
>>
>>Hi bionetter,
>>
>>  I am using a dye named SYBR Green I with no good results.
>>  I have heard that this dye make a better contrast in the UV light and 
>>then better sensitivity in nucleic acids agarose gel electroforresis  but 
>>a have amorphus (abnormal morphology) of the bands.
>>  I do the electroforetic run at:  100volt/300mA/50W and 1.2 Sigma 
>>agarose for a PCR product of 207 bp.  Using TBE 0.5X
>>  I use 1.5 microliter of green per 100ml of agarose
>>
>>This conditions have work for a long time using Etidium bromide but not 
>>with this dye.  
>>The test using higher quantitiest increase the problem
>>
>>       am I using the wrong buffer for this dye? 
JIM



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