SYBR Green I Dye for agarose gel eletroph. of DNA?
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Wed Nov 29 11:50:17 EST 1995
In article <49fesl$51c at news.acns.nwu.edu>, tar205 at nwu.edu (Tracey Ann Ristow Lozano) says:
>In article <Pine.A126.96.36.1991125154854.79008B-100000 at cariari.ucr.ac.cr>,
>japalaci at cariari.ucr.ac.cr says...
>> I am using a dye named SYBR Green I with no good results.
>> I have heard that this dye make a better contrast in the UV light and
>>then better sensitivity in nucleic acids agarose gel electroforresis but
>>a have amorphus (abnormal morphology) of the bands.
>> I do the electroforetic run at: 100volt/300mA/50W and 1.2 Sigma
>>agarose for a PCR product of 207 bp. Using TBE 0.5X
>> I use 1.5 microliter of green per 100ml of agarose
>>This conditions have work for a long time using Etidium bromide but not
>>with this dye.
>>The test using higher quantitiest increase the problem
>> am I using the wrong buffer for this dye?
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