pyronin y----acid buffer dye front

Dirk Seegert seegert at ita.fhg.de
Wed Nov 29 11:44:49 EST 1995


Hi Dave,
when we are making band shift assays we use "normal" sample buffer for 
agarose gels (0.25% bromphenolblue, 0.25% xylenecyanide, 30% glycerol) as 
front marker in an extra lane. Our (colorless) 5 x loading buffer for 
protein/dna complexes is made as follows: 200 mM KCl, 5 mM MgCl2, 0.5 mM 
EGTA, 2.5 mM DTT, 100 mM Hepes (pH 7.9) and 50% Glycerol.
Perhaps you should try this, may be it works.

Best regards

Leo




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