Microcircle Ligation

Todd Billeci billeci at itsa.ucsf.edu
Thu Nov 30 02:27:22 EST 1995


I am interested in ligating a DNA oligo that is annealed to a small 
circular DNA to give a double-stranded microcircle.

Would anyone have advice for optimizing such a ligation, reagents, 
enzymes, buffers, prayers?

Thanks.

Todd Billeci
University of California San Francisco
http://picasso.ucsf.edu/~billeci



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