Phosphorimager vs. Film

[Neil M Matsui]

On 30 Nov 1995, Eric C. Anderson wrote:
> In article <ege-3011951122280001 at cancer-ctr-mac-24.mit.edu>, ege at mit.edu
> (ege) wrote:
> 
> > Does anyone have a reliable figure for how sensitive a phosphorimager
> > screen is vs standard X ray film?  I seem to remember that manufacturers
> > claim 20X greater sensitivity then film but in my experience this figure
> > seems inflated.
> 
> it depends on the phosphorimager.  i don't know the figures for the Fujix
> BAS1000 since i only recently started using it, but the Molecular Dynamics
> one claimed about 10:1.  we did our own guesstimates that said it was more
> like 5-6 times faster.  the best thing about a phosphorimager besides the
> time savings though is the ease of quantitation compared with either
> scanning a film or using a densitometer.
> 
> eric
> 
> -- 
> Eric C. Anderson
> Cell Biology and Genetics
> Memorial Sloan-Kettering Cancer Center
> Sloan-Kettering Institute
> 1275 York Ave., Box 470
> New York, NY  10028
> Phone: (212)639-2977
> Fax:   (212)717-3298
> e-anderson at ski.mskcc.org

I have found similar time scale differences (about 5 times faster) with
that machine.

Neil

Neil M. Matsui
Cancer Research Institute
University of California, San Francisco
nmatsui at itsa.ucsf.edu
http://rafael.ucsf.edu/nmatsui/Tumorhome.html