phospholipids quantification?

H.J. Treumann Biochemistry ext 4301 hjtreuma at
Thu Nov 30 05:09:08 EST 1995

An elegant way of identifying and quantifying phospholipids from 
membrane extracts is electropsray mass spectrometry. A very good
reference where this has been done is:

Kerwin et al. (1994), Journal of Lipid Res. 35, pp. 1102-1114,
Identification of molecular species of glycerophospholipids and
sphingomyelin using electrospray mass spectrometry. 

This method is extremely sensitive and you can go down to the 
femtogram range if you optimise everything. 

email me if you have more questions about this. 


: > Anyone knows how can separate and quatify phospholipids from
: > erytrocyte membrane lipids using HPLC or any other methods?
Achim Treumann				tel. +44-1382-344301
Carbohydrate Research Centre		fax  +44-1382-322583
Department of Biochemistry
Dundee DD1 4HN, Scotland, UK		email a.treumann at

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