An elegant way of identifying and quantifying phospholipids from
membrane extracts is electropsray mass spectrometry. A very good
reference where this has been done is:
Kerwin et al. (1994), Journal of Lipid Res. 35, pp. 1102-1114,
Identification of molecular species of glycerophospholipids and
sphingomyelin using electrospray mass spectrometry.
This method is extremely sensitive and you can go down to the
femtogram range if you optimise everything.
email me if you have more questions about this.
: > Anyone knows how can separate and quatify phospholipids from
: > erytrocyte membrane lipids using HPLC or any other methods?
Achim Treumann tel. +44-1382-344301
Carbohydrate Research Centre fax +44-1382-322583
Department of Biochemistry
Dundee DD1 4HN, Scotland, UK email a.treumann at dundee.ac.uk