Duke Groebe (drg at prophet.pharm.pitt.edu) wrote:
: I'm attempting to precipitate short DNA oligos (68-mer) from a solution
: containing 7 M urea. I added glycogen to 30 ug/ml, Na acetate to 0.3 M,
: and 3 volumes of ethanol. I got a goopy clear ppt upon centrifugation and
: washing that I suspect is residual urea. A review of relevant literature
: was not very helpful. Any suggestions/insights that might help me
: resolve/avoid this situation would be greatly appreciated. Respond here or
: by e-mail (drg at prophet.pharm.pitt.edu).
If you think it might be the Urea that's affecting your pptation, why not
dialyze it away? Try dialyzing vs TE 4 or 5 times. Maybe more. The
concentration of urea in your solution is pretty high.
C A N A D A
Program in Molecular Biology
Department of Chemistry and Biochemistry Box 3C
New Mexico State University
Las Cruces NM