IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

Differential Display

Shahram Mori smori at nmsu.edu
Wed Nov 29 21:39:34 EST 1995

@ wrote:
: I have been doing differential display reactions and get good gels and can
: even amplify the bands out of the gel , But when I do northerns with these
: reamplified bands there isn't any signal at all on the blots. I know my
: RNA is fine and the transfer is fine too. Can't figure out what the
: problem is. 
: Any help would be really appreciated.
: Thanks,
: Aardraa Potnis
: apotnis at aim.salk.edu

This is what's been ruining my life for a year and a half. I get the 
exact same thing. *they* say that Northerns blots are not a good thing.
Some people say to clone and sequence first (because you might be looking 
at a lot more than one sequence) and then do northerns.
Others say do RPA's first....... BUT I don't see why northerns don't

p.s. I will bet that this method is MAINLY luck and has nothing to do with
consistent protocols.
			       _\  /_
			       \_  _/
			C   A   N   A   D   A
Shahram Mori
Program in Molecular Biology
Department of Chemistry and Biochemistry Box 3C
New Mexico State University
Las Cruces NM

More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net