Tim Angelotti (102055.606 at compuserve.com) wrote:
: I have heard about microcentrifuge spin columns that can be used to purify DNA from agarose
: gels. These are not based on DNA binding matrix in the spin-column but are merely a rapid spin
: that drives the gel solution and DNA out of the matrix. Does anyone know who sells these or
: how to make your own?
I think what all these kit people claim, is a bunch of lies. The procedures
look WAY too easy to get that good of DNA Yields. DNA. The best method in my
opinion is still the old-fashioned NaI/NaSO4. I tried Millipore's *columns*
and let me tell you that I would have gotten more DNA if I held the agarose
gel between my thumb and my index finger and squeezed!!!!
C A N A D A
Program in Molecular Biology
Department of Chemistry and Biochemistry Box 3C
New Mexico State University
Las Cruces NM