transfection of macrophages

Dirk Seegert seegert at
Wed Nov 29 08:05:33 EST 1995

Is anybody able to give hints for an effective transfection of primary 
macrophages and macrophage cellines? In the moment we are using the 
DEAE-dextrane method. Although changing a lot of parameters, we didn't 
have any success until now. Additionally we think about using other 
methods like electroporation or lipofectin. Does anybody have experience 
in this??
We would be glad about every good idea (or protocol?)

Best regards
Dr Dirk Seegert (nickname: Leo)
Fraunhofer Institute Hannover

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