5' RACE-Tail cDNA with dC, or dT?

Garry P. Larson glarson at ccmail.llu.edu
Mon Oct 2 20:26:53 EST 1995


Short and simple: The original 5' RACE technique tailed the first strand
cDNA with dATP using terminal transferase; however, other techniques employ
dCTP.  Is one better than the other?  If you've performed the technique,
please let me know what your preferences are.  Also, do you typically use a
nested downstream primer approach or can I just use my degenerate oligo for
the original PCR in combination with a d(N)n-adaptor primer (whatever "N"
might be)?

Thank you
GLarson



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