ABI automated sequencer

Dale Levitzke Dlevitzke at medicine.newcastle.edu.au
Tue Oct 3 01:37:29 EST 1995


I have acheived good results when sequencing PCR products by following 
this simple rule:
For each 300bp of PCR product to be sequenced use 100ng of template.
If for example you had 500bp of product to be sequenced I would round it 
up to the nearest figure, eg 200ng of template.  I always use 0.8 pmole 
of primer per reaction.

Good luck.





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