gel-shift problem

Eric Verdin verdin at
Wed Oct 4 15:26:58 EST 1995

The mobility of a DNA/protein complex in a gel shift is 
dependent on both the size of the bound protein and the charge 
of the protein.It is thus possible that your protein is bound 
to DNA but does not induce a shift because it is at its 
isoelectric point. To test this possibility, I would repeat 
the band shift using a buffer for the gel with a different pH.
Good luck 

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