transform bacteria with linear DNA?

Hank Seifert hseifert at casbah.acns.nwu.edu
Thu Oct 5 10:32:02 EST 1995


In article <105039758wnr at genesys.demon.co.uk>, 
Duncan at genesys.demon.co.uk says...
>
>We recently did some USE mutagenesis and wondered how efficiently 
>linearised plasmid transformed into E.coli relative to supercoiled. We used 
>the host TOP10 r-m- recA mcr- (Invitrogen) and in three independant 
>electroporations on different days ('cos we didn't believe the 
>results) found that linear plasmid electroporated at only roughly 10-3 down 
>on supercoiled. This was much higher than we expected.
>
>Duncan
Do you know that this was linear DNA and not uncut supercoiled?
-- 
Hank Seifert <h-seifert at nwu.edu>
Associate Professor
Department of Microbiology-Immunology
Searle 6-467, Maildrop W213
Northwestern University
303 East Chicago Ave.
Chicago, IL 60611
Tel: (312) 503-9788
Fax: (312) 503-9787 or -1339




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