PCR with 7-deaza analog
palasj at essex.UCHSC.edu
Fri Oct 6 16:25:13 EST 1995
We have found that using 7-deaza dGTP in the PCR reaction has worked
well to amplify a specific segment of DNA that has given us problems
otherwise. A concern that we have is that the incorporation of the
7-deaza dGTP will affect translation when the DNA segment is overexpressed.
Does anyone have experience in using this base analog in
PCR and using the product for cloning?
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