PCR with 7-deaza analog

Jeff Palas palasj at essex.UCHSC.edu
Fri Oct 6 16:25:13 EST 1995


We have found that using 7-deaza dGTP in the PCR reaction  has worked 
well to amplify  a specific segment of DNA that has given us problems 
otherwise. A concern that we have is that the incorporation of the 
7-deaza dGTP will affect translation when the DNA segment is overexpressed. 
Does anyone have experience in using this base analog in 
PCR and using the product for cloning?

Thanks

Jeff Palas
MYCOTOX,INC.
  



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