PCR with 7-deaza analog

Jeff Palas palasj at essex.UCHSC.edu
Fri Oct 6 16:25:13 EST 1995

We have found that using 7-deaza dGTP in the PCR reaction  has worked 
well to amplify  a specific segment of DNA that has given us problems 
otherwise. A concern that we have is that the incorporation of the 
7-deaza dGTP will affect translation when the DNA segment is overexpressed. 
Does anyone have experience in using this base analog in 
PCR and using the product for cloning?


Jeff Palas

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