Mutation rate in M13

Karl Fischer kfischer at gpu.srv.ualberta.ca
Fri Oct 6 15:23:13 EST 1995


In article <4540pe$6uf at masala.cc.uh.edu>, benedik at uh.edu (benedik at uh.edu) wrote:

> Does anyone know the relative mutation rate of things cloned in m13
> relative to a plasmid. I would have guessed that M13 has a high
> mutation rate, but I don't actually know that for sure. 

To the best of my knowledge, M13 DNA replication utilizes the host's DNA
polymerase I (E. coli Dpol I) so the mutation rate seen in this phage
should be a reflection of the misincorporation rate of this enzyme.

If I'm off base on this PLEASE correct me.

Karl

-- 
Karl Fischer
tyr-2 at bones.biochem.ualberta.ca
kfischer at gpu.srv.ualberta.ca




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