Storing Northerns

Tracy Aquilla aquilla at salus.med.uvm.edu
Wed Oct 4 07:40:57 EST 1995


In Article <e-anderson-2709951107330001 at e-mac-56.ski.mskcc.org>,
e-anderson at ski.mskcc.org (Eric C. Anderson) wrote:
>dear bionet-folk:
[snip]
>in the past, i have stored dried, unprobed northerns at -20oC in Saran
>Wrap or Seal-a-meal bags.  they worked fine after as long as 6 months (the
>longest i ever tried) or storing them.  but these were blots using yeast,
>C. elegans or mouse RNA, not exactly precious and easily re-made if
>necessary.

I have stored dry Northern blots in a drawer at room temp for several years
with no problems. I don't think it's necessary to store them at -20C.
I do keep them between sheets of filter paper just to keep them clean.
    Tracy



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