DNA degradation

CDMENU at ccs1.cc.monash.edu.au CDMENU at ccs1.cc.monash.edu.au
Sat Oct 7 01:57:50 EST 1995

Hi Netters,
l routinely use the miniprep method by Applied Biosystems Inc., since this 
method is supposed to give really clean DNA that can be used for sequencing. l 
have never had any problem with DNA degradation, however, recently, every time 
l use the DNA obtained using this method in restriction digests, the DNA 
degrades (l see a smear on the gel). l know that the degradation is not due to 
the plasmid construct, because l have used DNA obtained using a different 
method in digests and the DNA does not degrade. Has anyone had a similar 
problem, and if so, how did you solve it? Thanks in advance!


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