Which exonuclease can cut a 5' overhang?
"Marianne Leverone ", BIO
leverone at CHUMA.CAS.USF.EDU
Mon Oct 9 13:17:52 EST 1995
Laurentiu: ExoIII will chew off at 5' overhangs but not at 3' overhangs.
Marianne Leverone, Univ. of South Fla., Tampa, FL
On 2 Oct 1995, Laurentiu COCEA wrote:
> Hi- I have an XbaI 5' overhang and I have tried to digest it with
> Mung Bean Nuclease under normal conditions (NEB). Apparently, the
> ligation to a blunt fragment I attempted afterwards did not work.
> I suppose the next thing to do is try to use S1 nuclease instead of
> MBNase. But before I try it, I would greatly appreciate any suggestion
> on how I can blunt a 5' or 3' overhang without filling the recessed
> strand (in the former case) with nucleotides; that is, by a exonuclease
> treatment. Thank you very much for your help.
> Laurentiu COCEA
> MD., PhD. student
> INSERM U373, Institut Necker, Paris
> Tel. (33 1) 40 61 53 84
> Fax. (33 1) 40 61 55 90
> Internet: cocea at citi2.fr
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