Change in LB recipe?

"Marianne Leverone ", BIO leverone at CHUMA.CAS.USF.EDU
Mon Oct 9 12:50:43 EST 1995


Rafael:  I think that the additional salt improves plaque formation but I 
don't know why.  Marianne, Univ. of South Florida, Tampa, Fl.

On Thu, 5 Oct 1995, Rafael Maldonado wrote:

> On 5 Oct 1995, Dale Yuzuki's Work Account wrote:
> > 
> > Recently someone in our lab noticed that the premixed LB broth powder from
> > Difco contained 0.5 g/L NaCl instead of the 5.0 g/L formerly (also found
> > in Current Protocols and Sambrook). Maniatis' recipe had 10 g/L.
> 
> Don't be confused. Check "Experiments in Molecular Genetics", J.H. 
> Miller (1972) CSHL Press, and you will see the difference: 
> 
> Luria-Bertani Medium (LB)
> 
> 	NaCl	5 g/l  (10g/l in 1992 edition, called "A short 
> 			course in bacterial genetics", same author 
> 			and publisher, as in Maniatis.)
> 
> Luria Broth (also LB)
> 
> 	NaCl   0.5 g/l
> 
> So you can have Luria-Bertani broth (LB broth), or Luria Broth (LB), but 
> never both Luria Broth broth... With regarding to use 5 or 10 g/l, I don't 
> think that is going to be a big difference.
> 
> Luria-Bertani is the most used medium, and I think it would be fine for 
> most pourposes.
> 
> 
> 	-Rafa
> 
> 
> ___________________________________________________________________
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> Rafael Maldonado                             |  La cita ha sido
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