mini-preps: NOT kits.

gustilo at pobox.upenn.edu gustilo at pobox.upenn.edu
Mon Oct 9 13:04:28 EST 1995


In article <gustilo-0710952201520001 at ts11-14.upenn.edu>,
gustilo at pobox.upenn.edu wrote:

> ABI has a user bulletin on how to go about doing a plasmid prep for their
> automated sequencer. I believe it is User Bulletin #8.

sorry to follow up on my own posts but it is actually User Bulletin #18
not #8. Apologies for any inconvenience

> It is essentially an alkaline lysis prep as described in Promega's User
> Manual with the addition of a 13% PEG 8000 precipitation. They also
> recommend using Terrific Broth in place of LB to grow your overnights to
> increase the DNA yield using the same volumes.
> 
> --
> 
> Bicycle Crash Test Dummy for Hire
> gustilo at pobox.upenn.edu

--

Bicycle Crash Test Dummy for Hire
gustilo at pobox.upenn.edu



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