Direct sequencing from Lambda?

Sasha Kraev kraev at bc.biol.ethz.ch
Tue Oct 10 12:06:06 EST 1995


We use cycle sequencing kits from Epicentre Tech and lambda DNA,
prepared from small scale liquid lysates as in the Red Book.
Make sure your primers are >24 bases long and have
annealing temp ca. 70 C. 33P-labelled primers with 100 ng of
lambda give really high quality sequence.
Cheers,
Sasha





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