tat western blots

Mr P. Murphy pmurphy at liverpool.ac.uk
Mon Oct 9 07:54:53 EST 1995


I wish to detect HIV tat protein in Western blots.

I have tried Nitrocellulose and PVDF membranes

I have tried various antibodies from:

		JOHN KARNS at the HIV reagents repsitory
		Intracel
		Agmed

without notable success :-(

Can anyone advise me on a combination of membrane and primary antibody
which works?

I dont regularly scan these pages so if you could email me (pmurphy at liv.ac.uk)
I would be grateful.

Paul.



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