Linear acrylamide method

Duncan Clark Duncan at genesys.demon.co.uk
Tue Oct 10 04:10:30 EST 1995


Linear polyacrylamide is made by polymerising a 5% acrylamide 
solution in 40mM Tris, 20mM Na acetate, 1mM EDTA pH7.8 together with 
1/100 vol of 10% ammonium persulphate and 1/1000 vol. TEMED. When the 
solution becomes viscous (about 15-30mins) ppt polymer with 2.5volumes 
EtOH, centrifuge and redissolve pellet in 20 vol. water O/N. Use 10ul 
(25ug) per EtOH pptation.  

Duncan
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