Geneclean II Kit?

Hanbok Kim hbkim at biochem.kaist.ac.kr
Tue Oct 10 21:07:25 EST 1995


We use Geneclean II Kit (Bio 101) to purify DNA fragments.
Recovery is ok even though TBE buffer is used. The problem
is that it is very hard to subclone when the kit was used.
There might be some inhibitory effects on ligation.  
Agarose of Sigma (A-9539) is used. Is it not adequte?
Additionaly, do somebody know ingredients of TBE MODIFIER?
I would appreciate your advices.



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