Antibody purification questions
yatsen at wam.umd.edu
Wed Oct 11 21:56:28 EST 1995
I am planning to purify polyclonal antibody from rabbit serum.
The antigen is a recombinant poly His tagged protein. My current though
is to use the poly His tagged protein to make an affinity column to purify
the specific antibodies. I am pounded by the question that how I am going to
elute the antibodies from the column after washes without eluting the
I hope those who had tried this can give me your advices. I would
appreciate it a lot if you could share your protocol with me as well.
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