Mutator strains of E. coli

David de Graaf BMGraaf at dapsas1.weizmann.ac.il
Wed Oct 11 15:30:36 EST 1995


In article <4566c5$chu at newsbf02.news.aol.com> R Daines, rdaines at aol.com
writes:
>Looking for someone with experience with mutator strains of E. coli (ex. 
>Statagene cat# 2000129) to give me an idea of how well they work and with
>what frequence mutations are seen.
>
>
>Thanks in advance.
>Richard Daines


I used a MutD5 strain, described in the literature, becaue it does not
have as
may frameshists as the stratagene clone. I obtained about 1 mutation
every 800
bp after overnight culture. COnditions were a little tricky to set up,
but it
got me my PhD ;-)

David

A great many people think they are thinking when they are merely
rearranging their prejudices.
-- William James



More information about the Methods mailing list