sabine.hanelt at medizin.uni-ulm.de
Wed Oct 11 11:16:57 EST 1995
I'm working with the multiplex PCR to amplify the 9 exons of the HPRT gene.
One of our problems that occures from time to time is, that the larger
fragments (4 of 8) are very thin or not visible at all. I desided that there
has to be a contens with the nucleotides we are using, e.g. that they are to
old or frozen and thawn too often. But seemed not to be the only reason. The
multiplex showed better results, but not fantastic ones. I had to use double
of the amount of these primers I had used before and then the results were
Does anyone know this problem that only the larger bands get lost and has
anyone any other good idea than doubling the amount of primer.
And another question in conection with this is, how do you store your primers
and did you make any experiences that freezing and thawing of primers very
often reduces the quality of these primers?
Well, thank's a lot for your help
Abt. Medizinische Genetik
Uni Klinik Ulm
More information about the Methods