help with isoelectric focusing gels

Mr. J Hinshelwood jhinshel at
Wed Oct 11 12:21:11 EST 1995

Could someone suggest a protocol for running isoelectric gels. I intende 
to cast my own gel, using ampholytes, in a mini gel system. I am looking 
for a recipie for the gel, using bis-acrylamide,  and for the composition of 
the electrode buffers. 

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