"Marianne Leverone ", BIO
leverone at CHUMA.CAS.USF.EDU
Thu Oct 12 09:10:44 EST 1995
Hanbol Kim: Plasmid multimers are produced in a RecA+ host so use a
RecA- host. If you still see multimers after restriction digestion, your
digestion is not complete; add more enzyme and incubate and see if that
eliminates the problem. Marianne Leverone, Univ. of South Florida,
On 12 Oct 1995, Hanbok Kim wrote:
> After I moved to a different research place, one
> phenomenon bothers me. Intact plasmids before
> cutting with restriction enzymes are located in
> a 20 kb position, although its real size is 2.9 kb.
> It is pUC18. After cutting, the plasmid is seen at
> the right position. However a complete digestion is
> hard, and some mutimers (?) still remain in upper
> position. How can I prevent plasmid multimer?
> I would appreciate your advices.
> hbkim at biochem.kaist.ac.kr
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